Manipulating the Mouse Embryo: A Laboratory Manual

This edition offers a compilation of contemporary protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embyro and gamete cryop-reservation and rederivation, and gene expression. There is extensive background information on the use of these techniques. The "gold standard" techniques from previous editions for applying recombinant DNA technology to studies of mammalian embryonic development are also updated and recase, as is the summary of mouse development at a molecular level. This book offers a useful source of technical and theoretical guidance for mouse developmental biologists and geneticists and newcomers to these fields.